| Gene ID |
9255 |
| Accession |
Q12904 |
| Alternative Name |
EMAP II, EMAP-2, Aminoacyl tRNA synthase complex-interacting multifunctional protein 1, Multisynthase complex auxiliary component p43, Small inducible cytokine subfamily E member 1, SCYE1<br/>Recombinant Human Endothelial Monocyte-Activating Polypeptide 2 (AIMP1) |
| Species |
Human |
| Source |
E. coli |
| Description |
EMAP-II is activated during apoptosis by cleavage of pro-EMAP through caspase 8 and enhances the recruitment of inflammatory cells by its chemotactic properties. Besides its chemotactic effect on inflammatory cells, EMAP-II increases the adhesiveness of coronary artery smooth muscle cells (CASMC) for monocytes. EMAP-II also has anti-angiogenic properties, which are explained by its pro-apoptotic effect on endothelial cells. |
| Accession |
Q12904 |
| Functions |
The ED(50) was determined by apoptotic effect on MCF-7 cells using a concentration of 10-50 ng/mL. |
| Formulation |
Recombinant EMAP-II was lyophilized from a 0.2 ?m filtered 20 mM PB,130 mM NaCl solution pH 7.5. |
| Solubility |
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Appearance |
Lyophilized Powder |
| Molecular Weight |
18 |
| Purity |
>95% as determined by SDS-PAGE |
| Concentration |
<1.0 EU/μg of recombinant protein as determined by the LAL method. |
| Shipping Condition |
Ambient Temperature |
| Storage Condition |
The lyophilized protein is stable for at least one year from date of receipt at -70?C. Upon reconstitution, this cytokine can be stored in working aliquots at 2? – 8?C for one month, or at -20?C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
