Uric acid can be used as an antioxidant to remove peroxide, hydroxyl and oxygen free radicals, chelate and transfer metal ions, protect vascular endothelial cells from damage. Uric Acid in protein-free filtrate reduce phosphotungstic acid to form tungsten blue, allantoin and carbon dioxide, the depth of blue color is proportional to the concentration of uric acid.
Reagents
Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)
Labware
Vortex mixer, Micropipettor, Centrifuge
Size
100Assays
Sensitivity
0.58 mg/L
Detection Range
0.58-100 mg/L
Recovery Rate
105
Inter CV
2.6
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