Showing 46–54 of 65 results
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Recombinant Human AMPK (A2/B2/G2) (N-GST/C-His tag)
Product Details
Gene ID N/A Accession NM_006252, NM_005399, NM_001040633 Alternative Name Subunit A2: PRKAA2, AMPK, AMPK2, PRKAA Subunit B2: PRKAB2, MGC61468 Subunit G2: PRKAG2, AAKG, CMH6, WPWS, AAKG2, H91620p Species Human Source Insect cells Description AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis . AMPK exists as a heterotrimeric complex composed of a catalytic alpha subunit and regulatory beta and gamma subunits. Binding of AMP to the gamma subunit allosterically activates the complex. AMPK is activated in response to stresses that deplete cellular ATP (low glucose, hypoxia and ischemia) and via signaling pathways in response to adiponectin, leptin and CAMKKbeta. Accession NM_006252, NM_005399, NM_001040633 Functions The specific activity of AMPK was determined to be 65 nmol /min/mg as per activity assay protocol. Formulation 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. Solubility N/A Appearance Liquid Molecular Weight 92 Purity 70% – 90% Shipping Condition Dry Ice Storage Condition Store product at ?70?C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. $0.00(No) -
Recombinant Human AMPK (A2/B2/G3) (N/C His tag)
Product Details
Gene ID N/A Accession NM_006252, NM_005399, NM_017431 Alternative Name Subunit A2: PRKAA2, AMPK, AMPK2, PRKAA Subunit B2: PRKAB2, MGC61468 Subunit G3: PRKAG3 Species Human Source Insect cells Description AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis . AMPK exists as a heterotrimeric complex composed of a catalytic alpha subunit and regulatory beta and gamma subunits. Binding of AMP to the gamma subunit allosterically activates the complex. AMPK is activated in response to stresses that deplete cellular ATP (low glucose, hypoxia and ischemia) and via signaling pathways in response to adiponectin, leptin and CAMKKbeta. Accession NM_006252, NM_005399, NM_017431 Functions The specific activity of AMPK was determined to be 58 nmol /min/mg as per activity assay protocol Formulation 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. Solubility N/A Appearance Liquid Molecular Weight 92 Purity 70% – 90% Shipping Condition Dry Ice Storage Condition Store product at ?70?C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. $0.00(No) -
Recombinant Human ANKK1 (SGK288) (N-GST tag)
Product Details
Gene ID N/A Accession NM_178510 Alternative Name X-kinase, PKK2, SGK288 Species Human Source Insect cells Description ANKK1 or ankyrin repeat and kinase domain containing 1 is belongs to the Ser/Thr protein kinase family, and protein kinase superfamily that involved in signal transduction pathways . ANKK1 is closely linked to DRD2 gene on chr 11, and a well studied restriction fragment length polymorphism (RFLP) designated TaqIA, was originally associated with the DRD2 gene and it was determined to be located in exon 8 of ANKK1 gene where it causes a nonconservative amino acid substitution . Accession NM_178510 Functions The specific activity of ANKK1 (SGK288) was determined to be 80 nmol/min/mg as per activity assay protocol Formulation 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. Solubility N/A Appearance Liquid Molecular Weight 115 Purity 70% – 90% Shipping Condition Dry Ice Storage Condition Store product at ?70?C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. $0.00(No) -
Recombinant Human AREG
Product Details
Gene ID 374 Accession P15514 Alternative Name AR, Colorectum cell-derived growth factor, CRDGF<br/>Recombinant Human Amphiregulin (AREG) Species Human Source E. coli Description Amphiregulin is a trans-membrane protein whose protease mediated digestion of the extracellular domain enables release of mature AR. Amphiregulin works to stimulate the proliferation of some animal keratinocyles, epithelial cells and fibroblasts. It has been shown that the mRNA expression of amphiregulin can be detected in numerous carcinoma cell lines and the epithelial cells of various human tissues, including colon, stomach, breast, ovary and kidney. Accession P15514 Functions Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED(50) for this effect is typically 5?15 ng/mL. Formulation Lyophilized from a 0.2 ?m filtered solution in PBS with BSA as a carrier protein Solubility Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin Appearance Lyophilized Powder Molecular Weight 11.5 Purity >95% as determined by SDS-PAGE Concentration <1.0 EU/μg of recombinant protein as determined by the LAL method. Shipping Condition Ambient Temperature Storage Condition The lyophilized protein is stable for at least one year from date of receipt at -70?C. Upon reconstitution, this cytokine can be stored in working aliquots at 2? – 8?C for one month, or at -20?C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. $0.00(No) -
Recombinant Human AREG
Product Details
Gene ID 374 Accession P15514 Alternative Name AR, Colorectum cell-derived growth factor, CRDGF<br/>Recombinant Human Amphiregulin (AREG) Species Human Source E. coli Description Amphiregulin is a trans-membrane protein whose protease mediated digestion of the extracellular domain enables release of mature AR. Amphiregulin works to stimulate the proliferation of some animal keratinocyles, epithelial cells and fibroblasts. It has been shown that the mRNA expression of amphiregulin can be detected in numerous carcinoma cell lines and the epithelial cells of various human tissues, including colon, stomach, breast, ovary and kidney. Accession P15514 Functions Measured in a cell proliferation assay using mouse embryonic fibroblast 3T3 cells. The ED(50) for this effect is <0.5 ng/mL. Formulation Lyophilized from a 0.2 ?m filtered solution in PBS with BSA as a carrier protein Solubility Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin Appearance Lyophilized Powder Molecular Weight 11.5 Purity >95% as determined by SDS-PAGE Concentration <1.0 EU/μg of recombinant protein as determined by the LAL method. Shipping Condition Ambient Temperature Storage Condition The lyophilized protein is stable for at least one year from date of receipt at -70?C. Upon reconstitution, this cytokine can be stored in working aliquots at 2? – 8?C for one month, or at -20?C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. $0.00(No) -
Recombinant Human Artemin (ARTN)
Product Details
Gene ID 9048 Accession Q5T4W7 Alternative Name Enovin, Neublastin Species Human Source E. coli Description Artemin is a disulfide-linked member of the glial cell line-derived homodimeric neurotrophic factor (GDNF) family which all signal via a multicomponent receptor system composing of a high-affinity binding component (GFR?1-4) and a common signalling receptor tyrosine kinase (RET). Artemin prefers the receptor GFR?3-RET, but will use the others as an alternative. Artemin promotes the survival and growth of peripheral and central neurons, including dopaminergic neurons in the mid-brain; and plays an important role in the development of sensory and sympathetic neurons. Accession Q5T4W7 Functions The ED50 as determined by its binding ability in a functional ELISA (using SH-SY5Y human neuroblastoma cells), is 4-16 ng/mL Formulation Lyophilized from a 0.2 ?m filtered solution in HCl (with BSA as a carrier protein) Solubility Reconstitute in sterile 4mM HCl at 100 μg/mL (containing at least 0.1% human or bovine serum albumin) Appearance Lyophilized Powder Molecular Weight 12.2 Purity >95% as determined by SDS-PAGE Concentration < 1.0 EU/μg of recombinant protein as determined by the LAL method. Shipping Condition Ambient Temperature Storage Condition The lyophilized protein is stable for at least one year from date of receipt at -70?C. Upon reconstitution, this cytokine can be stored in working aliquots at 2? – 8?C for one month, or at -20?C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. $0.00(No) -
Recombinant Human Artemin (ARTN)
Product Details
Gene ID 9048 Accession Q5T4W7 Alternative Name Enovin, Neublastin Species Human Source E. coli Description Artemin is a disulfide-linked member of the glial cell line-derived homodimeric neurotrophic factor (GDNF) family which all signal via a multicomponent receptor system composing of a high-affinity binding component (GFR?1-4) and a common signalling receptor tyrosine kinase (RET). Artemin prefers the receptor GFR?3-RET, but will use the others as an alternative. Artemin promotes the survival and growth of peripheral and central neurons, including dopaminergic neurons in the mid-brain; and plays an important role in the development of sensory and sympathetic neurons. Accession Q5T4W7 Functions The ED50 as determined by its binding ability in a functional ELISA (using SH-SY5Y human neuroblastoma cells), is 4-16 ng/mL Formulation Lyophilized from a 0.2 ?m filtered solution in HCl (with BSA as a carrier protein) Solubility Reconstitute in sterile 4mM HCl at 100 μg/mL (containing at least 0.1% human or bovine serum albumin) Appearance Lyophilized Powder Molecular Weight 12.2 Purity >95% as determined by SDS-PAGE Concentration <1.0 EU/μg of recombinant protein as determined by the LAL method Shipping Condition Ambient Temperature Storage Condition The lyophilized protein is stable for at least one year from date of receipt at -70?C. Upon reconstitution, this cytokine can be stored in working aliquots at 2? – 8?C for one month, or at -20?C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. $0.00(No) -
Recombinant Human ASK1 (N-GST tag)
Product Details
Gene ID N/A Accession NM_005923 Alternative Name MAP3K5; MEKK5; MAPKKK5 Species Human Source Insect cells Description ASK1, also known as MAPKKK5, activates MKK3, MKK4 (SEK1), and MKK6. Overexpression of ASK1 induces apoptotic cell death, and ASK1 is activated in cells treated with tumor necrosis factor-alpha . ASK1 interacts with members of the TRAF family and is activated by TRAF2 in the TNF-signaling pathway. After activation by TRAF2, ASK1 activates MKK4, which in turn activates JNK. Thus, ASK1 is a mediator of TRAF2-induced JNK activation . Accession NM_005923 Functions The specific activity of ASK1 was determined to be 97 nmol /min/mg as per activity assay protocol. Formulation 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol. Solubility N/A Appearance Liquid Molecular Weight 60 Purity 70% – 90% Shipping Condition Dry Ice Storage Condition Store product at ?70?C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. $0.00(No) -
Recombinant Human AURORA A (N-GST tag)
Product Details
Gene ID N/A Accession NM_003600 Alternative Name AURKA, AIK, ARK1, AURA, BTAK, STK6, STK7, STK15, AURORA2, MGC34538 Species Human Source Insect cells Description AURORA A belongs to a multigenic family of mitotic serine/threonine kinases which are involved in the control of chromosome segregation. AURORA A is involved in centrosome separation, duplication and maturation as well as in bipolar spindle assembly and stability . AURORA A is expressed and active at the highest level during G2-M phase of the cell cycle. Overexpression of AURORA A has been found to be correlated with the grade of various human solid tumours. Ectopic AURORA A overexpression in any culture cell line leads to polyploidy and centrosome amplification . Accession NM_003600 Functions The specific activity of AURORA A was determined to be 73 nmol /min/mg as per activity assay protocol Formulation 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol. Solubility N/A Appearance Liquid Molecular Weight 72 Purity 70% – 90% Shipping Condition Dry Ice Storage Condition Store product at ?70?C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. $0.00(No)