Showing 1–9 of 86 results
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Antibodies
Product Details
Product Name Histone H3(di methyl K9) Monoclonal antibody Cat Number HR1AM2219 Source Mouse Applications WB, IP, IHC Species Reactivity Human,Rat,Mouse Recommended Dilutions WB 1:1,000-3,000 IHC 1:200-500 Specificity Human Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles. Clonality Monoclonal Isotope IgG1 Concentration 1mg/ml Observed Band 15kDa Gene ID 8290 Alternative Name H3 histone antibody, HIST1H3A antibody, Histone cluster 1, H3a antibody Background Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Type monoclonal $0.00(3) -
Bioassay Kit
Product Details
Intrument Spectrophotometer(690 nm) Assay Time 60 min Validity 3 Assay Type Quantitative Sample Type Serum,plasma,urine Synonyms UA Detection Principal Uric acid can be used as an antioxidant to remove peroxide, hydroxyl and oxygen free radicals, chelate and transfer metal ions, protect vascular endothelial cells from damage. Uric Acid in protein-free filtrate reduce phosphotungstic acid to form tungsten blue, allantoin and carbon dioxide, the depth of blue color is proportional to the concentration of uric acid. Reagents Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4) Labware Vortex mixer, Micropipettor, Centrifuge Size 100Assays Sensitivity 0.58 mg/L Detection Range 0.58-100 mg/L Recovery Rate 105 Inter CV 2.6 $0.00(3) -
Biotinylation Kit
Product Overview
Biotin conjugation/ labeling in <20 mins with 30 secs hands-on time using Biotinylation Kit/ Biotin Conjugation Kit. Designed for full antibody recovery (100%).
Kit Components
1 mg Quencher reagent (1 x 200 µL); Biotin Conjugation Mix (1 x 1 mg); Modifier reagent (1 x 200 µL) 100 µg Biotin Conjugation Mix (1 x 100 µg); Modifier reagent (1 x 200 µL) 3 x 10 µg Biotin Conjugation Mix (3 x 10 µg); Modifier reagent (1 x 200 µL); Quencher reagent (1 x 200 µL) 3 x 100 µg Quencher reagent (1 x 200 µL); Biotin Conjugation Mix (3 x 100 µg); Modifier reagent (1 x 200 µL) Storage
Shipping Ambient – Cannot Ship with Ice Short-term storage -20ºC Long-term storage -20ºC Applications and Use
Biotinylation Kit/ Biotin Conjugation Kit uses a simple and quick process for biotinylation/ biotin labeling of antibodies. It can also be used to conjugate other proteins or peptides.
Add modifier to antibody and incubate for 15 mins. Add quencher and incubate for 5 mins. The conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.
The Biotinylation Kit/ Biotin Conjugation Kit is optimized to produce conjugates for assays in which the biotinylated protein is captured by streptavidin immobilized on a surface (e.g., plates, nitrocellulose, magnetic beads etc). Custom size conjugation kits up to 100 mg are available on demand.
Amount and volume of antibody for conjugation to Biotin
Kit Size Recommended amount Maximum amount Maximum antibody volume 3 x 10 μg 3 x 10 μg 3 x 20 μg 3 x 10 μL 100 μg 1 x 100 μg 1 x 200 μg 1 x 100 μL 3 x 100 μg 3 x 100 μg 3 x 200 μg 3 x 100 μL 1 x 1 mg 1 x 1 mg 1 x 2 mg 1 x 1 mL Using the maximum amount of antibody may result in less labelling per antibody. Ideal antibody concentration is 1 mg/ml. 0.5 – 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 2 mg/ml or < 0.5 mg/ml should be diluted /concentrated.
Buffer Requirements for Conjugation
Buffer should be pH 6.5-8.5. If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.
Compatible constituents 50mM / 0.6% Tris; 0.1% BSA; 50% glycerol; 0.1% sodium azide; PBS; potassium phosphate; sodium chloride; HEPES; sucrose; sodium citrate; EDTA; trehalose. Incompatible constituents Thiomerosal; Proclin; Glycine; Arginine; Glutathione; DTT. Tris buffered saline is almost always ≤ 50 mM / 0.6%. BSA can also interfere with the use of the conjugated antibody in tissue staining. If a constituent of the buffer containing your antibody or protein is not listed above, please contact us. Only purified antibodies are suitable for use, i.e. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.
Storing and handling conjugation kits
Lyophilized components are hygroscopic. Kits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.
For research use only, not for clinical diagnosis.
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Bovine Serum Albumin (BSA) Fraction V, Protease-free, Globulin-free (essentially), ≥98% Pure
Product Description: Bovine Serum Albumin (BSA) Fraction V, Protease-free, Globulin-free (essentially), ≥98% Pure
Product Overview
EvoBioSystems’ Protease-Free Bovine Serum Albumin (BSA) is a high-purity, heat shock–fractionated serum albumin designed for sensitive biochemical and analytical applications. BSA serves as a critical reagent for protein quantitation, immunoassays, cell culture, and enzyme-based workflows. This specialized grade is rigorously tested to ensure extremely low protease activity and minimal impurities, making it ideal for enzyme assays, nucleic acid hybridization, EIA, RIA, luminescence assays, and other protease-sensitive techniques. Its globulin-free profile, consistent performance, and reliable stability make it an essential component for research laboratories, diagnostic developers, and biopharma workflows.
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CD42b / GPIbα Antibody
Product Overview
This biotin-conjugated monoclonal antibody recognizes human CD42b, and is also known as platelet glycoprotein GPIbα (Glycoprotein Ib alpha). CD42b is expressed on the surface of human platelets and megakaryocytes. Clone AK2 has been reported to inhibit the interaction between von Willebrand factor (VWF) and platelet.
Clone AK2 Target CD42b / GPIbα Gene Symbol GP1BA Gene ID 2811 Species Reactivity Human Additional Reactivity Mouse reactivity has been reported in scientific literature (PMID: 29437579) Immunogen Human CD42b Unit Size 0.1 mL Concentration Refer to vial label Host Species Mouse Clonality Monoclonal Isotype IgG1 Conjugate Biotin Purification Protein G purified Buffer PBS Preservative 0.05% Sodium azide Storage Conditions Store at 4°C protected from light Specificity The antibody specifically recognizes the human CD42b cell surface antigen (platelet glycoprotein GPIbα), which is expressed on platelets and megakaryocytes. Application Recommended Use
Application Recommended Use ELISA ✓ Suitable Flow Cytometry ✓ Suitable Immunoprecipitation ✓ Suitable (No) -
CD61 / Integrin Beta 3 Antibody
Product Overview
Target Integrin Beta 3 (CD61 / GPIIIa / ITGB3) Conjugate Biotin Quantity 100 µg Volume 200 µL Host Immunoglobulin Rabbit IgG Clonality Polyclonal Purification Affinity purified Sodium Azide 0.02% Form Liquid Concentration 0.5–1.5 mg/mL IgG in antibody stabilization buffer (proprietary buffer, pH 7.4–7.8, with 0.5% BSA, 30% glycerol) Storage Conditions Store at −20°C for long-term storage Immunogen Synthetic peptide corresponding to amino acid region 1–50 of the human Integrin Beta 3 subunit protein. Species Reactivity Human and Monkey Application Recommended Dilution
Application Recommended Dilution ELISA 1:10,000 Western Blot (WB) 1:500–1:1,000 Immunohistochemistry (IHC) 1:150–1:250 Immunocytochemistry / Immunofluorescence (ICC/IF) 1:150–1:250 Immunoprecipitation (IP) 1:200 Note: Optimal antibody dilution may require further optimization depending on sample type and experimental conditions.
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Claudin-2 (8H11) Mouse mAb
S.No Query Isotype IgG1 Clonality Monoclonal Concentration 1 mg/ml Observed band 25kDa GeneID（Human） 9075 Human Swiss-Prot No. P57739 Cellular Localization Cell junctiontight junctionCell Membrane Background The claudin family is composed of 23 integral membrane proteins and their expression which varies among tissue types may determine both the strength and properties of the epithelial barrier. Alteration in claudin protein expression pattern is associated with several types of cancer . Claudin-2 is expressed primarily in the proximal tubule of the normal mammalian kidney where it regulates transepithelial ion (e.g. Na+ Cl-) reabsorption . Increased expression of Claudin-2 has been reported in some cancer cell lines $0.00(3) -
Cyclophosphamide Monohydrate
Product Description: Cyclophosphamide Monohydrate
Application Notes:
Cyclophosphamide monohydrate used for induction chemotherapy to study predictive and prognostic values of tumor MGMT gene expression, to study its effects on apoptosis and cell cycle progression and its cardiotoxicity during pretransplant conditioning in blood stem cell transplantation has been reported.
Usage Statement:
Unless specified otherwise, Evobio Sysytems products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications:
Cancer research tool
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Dye based RNA Quantification Kit
Product Details
- RNA quantification range: 1 ng/mL to 1000 ng/mL.
- Compatible with standard plate and cuvette fluorometers, as well as NanoDrop™ 3300 and readers versions 3.0 and 4.0.
RNA Quantification Reagent, 100 µL 1 RNA Quantification Standard, 100 ng/µL in TE Buffer, 100 µL 1 TE Buffer (RNase-free), 20x, 25 mL 1 $0.00(No)