Showing 1–9 of 59 results
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Antibodies
Product Details
Product Name Histone H3(di methyl K9) Monoclonal antibody Cat Number HR1AM2219 Source Mouse Applications WB, IP, IHC Species Reactivity Human,Rat,Mouse Recommended Dilutions WB 1:1,000-3,000 IHC 1:200-500 Specificity Human Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles. Clonality Monoclonal Isotope IgG1 Concentration 1mg/ml Observed Band 15kDa Gene ID 8290 Alternative Name H3 histone antibody, HIST1H3A antibody, Histone cluster 1, H3a antibody Background Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Type monoclonal ₹0.00(3) -
Bioassay Kit
Product Details
Intrument Spectrophotometer(690 nm) Assay Time 60 min Validity 3 Assay Type Quantitative Sample Type Serum,plasma,urine Synonyms UA Detection Principal Uric acid can be used as an antioxidant to remove peroxide, hydroxyl and oxygen free radicals, chelate and transfer metal ions, protect vascular endothelial cells from damage. Uric Acid in protein-free filtrate reduce phosphotungstic acid to form tungsten blue, allantoin and carbon dioxide, the depth of blue color is proportional to the concentration of uric acid. Reagents Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4) Labware Vortex mixer, Micropipettor, Centrifuge Size 100Assays Sensitivity 0.58 mg/L Detection Range 0.58-100 mg/L Recovery Rate 105 Inter CV 2.6 ₹0.00(3) -
Claudin-2 (8H11) Mouse mAb
S.No Query Isotype IgG1 Clonality Monoclonal Concentration 1 mg/ml Observed band 25kDa GeneID（Human） 9075 Human Swiss-Prot No. P57739 Cellular Localization Cell junctiontight junctionCell Membrane Background The claudin family is composed of 23 integral membrane proteins and their expression which varies among tissue types may determine both the strength and properties of the epithelial barrier. Alteration in claudin protein expression pattern is associated with several types of cancer . Claudin-2 is expressed primarily in the proximal tubule of the normal mammalian kidney where it regulates transepithelial ion (e.g. Na+ Cl-) reabsorption . Increased expression of Claudin-2 has been reported in some cancer cell lines ₹0.00(3) -
Elisa Kit
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiotensin I Converting Enzyme(ACE). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Angiotensin I Converting Enzyme(ACE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiotensin I Converting Enzyme(ACE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Angiotensin I Converting Enzyme(ACE) in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Details
Product Name Human ACE(Angiotensin I Converting Enzyme) ELISA Kit Cat No HR1E1111 Species Human Alternative Name CD143; ACE1; DCP1; ACEI; ACE-I; Kininase II; Angiotensin-Converting Enzyme; Peptidyl-Dipeptidase A; Dipeptidyl Carboxypeptidase 1; Angiotensin-converting enzyme, soluble form Sensitivity 0.16 ng/mL Standard 25 ng/mL Detection Range 0.4-25 ng/mL Assay Length 3.5h Research Area Enzyme & Kinase;Cardiovascular biology; Sample Type serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids ₹0.00(3) -
Favipiravir
S.No Query Molecular Formula C5H4FN3O2 Melting Point/Freezing Point >151°C (dec.) Molecular Weight 157.10 CAS Registry 259793-96-9 Synonyms 6-Fluoro-3,4-dihydro-3-oxo-2-pyrazinecarboxamide; 6-Fluoro-3,4-dihydro-3-oxo-pyrazinecarboxamide; 6-Fluoro-3-hydroxy-2- pyrazinecarboxamide; Favipiravir; T 705; T 705 (Pharmaceutical) Eye/Face Protection Safety goggles or face shield. All equipment should have been tested and approved under appropriate standards, such as NIOSH (US), CSA (Canada), or EN 166 (EU). ₹0.00(3) -
Hot Start Taq DNA Polymerase
Hot Start Taq DNA Polymerase does not require special high temperature treatment. The pre-denaturation step in conventional PCR reaction conditions can start the activity of Taq enzyme. It is suitable for various Taq enzyme-based hot-start PCR and qPCR reactions. The PCR product has an A at the 3’end, which can be cloned directly with TA vector.
Unit definition At 74°C for 30 min, the amount of enzyme required to incorporate 10 nm dNTPs into the acid-insoluble precipitate is defined as 1 activity unit. Activity detection conditions: 50 mM Tris-Hcl (pH 9.0, 25°C), 50 mM NaCl, 5 mM MgCl2, 0.2 mM each dNTPs (including [3H]-Dttp), 200 μg/ml activated calf thymus DNA and 0.1 mg/ml BSA.
Quality Control
The purity detected by SDS-PAGE is greater than 99%. After detection of no exogenous nuclease activity, PCR method detects no host DNA residues, which can effectively amplify single-copy genes in the human genome.
PCR system components
- 1. Purity of template DNA: Many residual nucleic acid extraction reagents will affect the PCR reaction, including protease, protein denaturant (such as SDS, guanidine salt), high concentration salt (KAc, NaAc, sodium caprylate, etc.) and high concentration EDTA. The amount of template with low purity (such as the template obtained by boiling method) should not exceed 1/10 of the PCR reaction system (for example, the volume of template added to the 50 μl reaction system should not exceed 5 μl). If the purity of the template DNA is too poor, you can use our PCR product recovery kit (Cat. No. EP005) to purify and concentrate the template DNA. The amount of template purified by our PCR product recovery kit can be as much as 1/2 of the volume of the PCR reaction system.
- 2. The amount of template DNA: a very small amount of DNA can also be used as a template for PCR, but to ensure the stability of the reaction, it is recommended to use target sequences with more than 104 copies as a template for a 50μl system. Recommended amount of template DNA.
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Human IL6 (Interleukin 6) ELISA Kit
Special Explanation
1. Store kit at 4°C immediately upon receipt.
2. Do not use the kit after the expiration date.
3. Please check whether all components are complete after opening the package.
All kit components have been formulated and quality control tested to function successfully as a kit. Do not mix or substitute reagents or materials from other kit, performance cannot be guaranteed if utilized separately or substituted.
Materials Required, Not Supplied
1. Microplate reader capable of measuring absorbance at 450 ± 10 nm.
2. High-speed centrifuge.
3. Electro-heating standing-temperature cultivator.
4. Absorbent paper.
5. Distilled or deionized water.
6. Single or multi-channel pipettes with high precision and disposable tips.
7. Precision pipettes to deliver 2 μL to 1 mL volumes.
Safety notes
1. This kit is sold for lab research and development use only and not for use in humans or animals.
2. Reagents should be treated as hazardous substances and should be handled with care and disposed of properly.
3. Gloves, lab coat, and protective eyewear should always be worn, Avoid any skin and eye contact with Stop Solution and TMB. In case of contact, wash thoroughly with water.
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Recombinant Human Adiponectin (Acrp30)
Product Details
Gene ID 9370 Accession Q15848 Alternative Name 30 kDa adipocyte complement-related protein, Adipocyte complement-related 30 kDa protein, Adipocyte, C1q and collagen domain-containing protein, Adipose most abundant gene transcript 1 protein, Gelatin-binding protein Species Human Source E. coli Description Adiponectin is an adipose-derived secreted protein that influences the body?s response to insulin. Adiponectin belongs to the soluble defence collagen super family and is involved with the promotion of adipocyte differentiation, fatty acid catabolism, and insulin resistance. Recombinant adiponectin is a multimeric glycoprotein that also has anti-inflammatory effects on the cell lining of blood vessel walls. High blood levels of adiponectin are associated with reduced risks of heart attacks while low levels are associated with obesity and an increased risk of heart attacks. Accession Q15848 Functions The ED50 , as determined by a cytotoxic assay (using M1 cells) was found to be 3.0-6.0 μg/ml Formulation Lyophilized from a 0.2 ?m filtered solution in PBS Solubility Reconstitute in sterile deionized water to 0.1 mg/mL Appearance Lyophilized Powder Molecular Weight 24.8 Purity >95% as determined by SDS-PAGE Concentration < 1.0 EU/μg of recombinant protein as determined by the LAL method. Shipping Condition Ambient Temperature Storage Condition The lyophilized protein is stable for at least one year from date of receipt at -70?C. Upon reconstitution, this cytokine can be stored in working aliquots at 2? – 8?C for one month, or at -20?C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. ₹0.00(No) -
Recombinant Human Adiponectin (Acrp30)
Product Details
Gene ID 9370 Accession Q15848 Alternative Name 30 kDa adipocyte complement-related protein, Adipocyte complement-related 30 kDa protein, Adipocyte, C1q and collagen domain-containing protein, Adipose most abundant gene transcript 1 protein, Gelatin-binding protein Species Human Source E. coli Description Adiponectin is an adipose-derived secreted protein that influences the body?s response to insulin. Adiponectin belongs to the soluble defence collagen super family and is involved with the promotion of adipocyte differentiation, fatty acid catabolism, and insulin resistance. Recombinant adiponectin is a multimeric glycoprotein that also has anti-inflammatory effects on the cell lining of blood vessel walls. High blood levels of adiponectin are associated with reduced risks of heart attacks while low levels are associated with obesity and an increased risk of heart attacks. Accession Q15848 Functions The ED50, as determined by a cytotoxic assay (using M1 cells) was found to be 3.0-6.0 μg/ml Formulation Lyophilized from a 0.2 ?m filtered solution in tris buffer with NaCl (pH 8) Solubility Reconstitute in sterile deionized water to 0.1 mg/ml Appearance Lyophilized Powder Molecular Weight 24.8 Purity >95% as determined by SDS-PAGE Concentration < 1.0 EU/μg of recombinant protein as determined by the LAL method. Shipping Condition Ambient Temperature Storage Condition The lyophilized protein is stable for at least one year from date of receipt at -70?C. Upon reconstitution, this cytokine can be stored in working aliquots at 2? – 8?C for one month, or at -20?C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. ₹0.00(No)