Intrument |
Spectrophotometer(690 nm) |
Assay Time |
60 min |
Validity |
3 |
Assay Type |
Quantitative |
Sample Type |
Serum,plasma,urine |
Synonyms |
UA |
Detection Principal |
Uric acid can be used as an antioxidant to remove peroxide, hydroxyl and oxygen free radicals, chelate and transfer metal ions, protect vascular endothelial cells from damage. Uric Acid in protein-free filtrate reduce phosphotungstic acid to form tungsten blue, allantoin and carbon dioxide, the depth of blue color is proportional to the concentration of uric acid. |
Reagents |
Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4) |
Labware |
Vortex mixer, Micropipettor, Centrifuge |
Size |
100Assays |
Sensitivity |
0.58 mg/L |
Detection Range |
0.58-100 mg/L |
Recovery Rate |
105 |
Inter CV |
2.6 |