Elisa Kit
Elisa Kit
Alternative Name: CD143; ACE1; DCP1; ACEI; ACE-I; Kininase II; Angiotensin-Converting Enzyme; Peptidyl-Dipeptidase A; Dipeptidyl Carboxypeptidase 1; Angiotensin-converting enzyme, soluble form
Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiotensin I Converting Enzyme(ACE). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Angiotensin I Converting Enzyme(ACE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiotensin I Converting Enzyme(ACE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Angiotensin I Converting Enzyme(ACE) in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Details
| Product Name | Human ACE(Angiotensin I Converting Enzyme) ELISA Kit |
| Cat No | HR1E1111 |
| Species | Human |
| Alternative Name | CD143; ACE1; DCP1; ACEI; ACE-I; Kininase II; Angiotensin-Converting Enzyme; Peptidyl-Dipeptidase A; Dipeptidyl Carboxypeptidase 1; Angiotensin-converting enzyme, soluble form |
| Sensitivity | 0.16 ng/mL |
| Standard | 25 ng/mL |
| Detection Range | 0.4-25 ng/mL |
| Assay Length | 3.5h |
| Research Area | Enzyme & Kinase;Cardiovascular biology; |
| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
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