Showing 10–18 of 142 results
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Hot Start Taq DNA Polymerase
Hot Start Taq DNA Polymerase does not require special high temperature treatment. The pre-denaturation step in conventional PCR reaction conditions can start the activity of Taq enzyme. It is suitable for various Taq enzyme-based hot-start PCR and qPCR reactions. The PCR product has an A at the 3’end, which can be cloned directly with TA vector.
Unit definition At 74°C for 30 min, the amount of enzyme required to incorporate 10 nm dNTPs into the acid-insoluble precipitate is defined as 1 activity unit. Activity detection conditions: 50 mM Tris-Hcl (pH 9.0, 25°C), 50 mM NaCl, 5 mM MgCl2, 0.2 mM each dNTPs (including [3H]-Dttp), 200 μg/ml activated calf thymus DNA and 0.1 mg/ml BSA.
Quality Control
The purity detected by SDS-PAGE is greater than 99%. After detection of no exogenous nuclease activity, PCR method detects no host DNA residues, which can effectively amplify single-copy genes in the human genome.
PCR system components
- 1. Purity of template DNA: Many residual nucleic acid extraction reagents will affect the PCR reaction, including protease, protein denaturant (such as SDS, guanidine salt), high concentration salt (KAc, NaAc, sodium caprylate, etc.) and high concentration EDTA. The amount of template with low purity (such as the template obtained by boiling method) should not exceed 1/10 of the PCR reaction system (for example, the volume of template added to the 50 μl reaction system should not exceed 5 μl). If the purity of the template DNA is too poor, you can use our PCR product recovery kit (Cat. No. EP005) to purify and concentrate the template DNA. The amount of template purified by our PCR product recovery kit can be as much as 1/2 of the volume of the PCR reaction system.
- 2. The amount of template DNA: a very small amount of DNA can also be used as a template for PCR, but to ensure the stability of the reaction, it is recommended to use target sequences with more than 104 copies as a template for a 50μl system. Recommended amount of template DNA.
$0.00(3) -
Human IL6 (Interleukin 6) ELISA Kit
Special Explanation
1. Store kit at 4°C immediately upon receipt.
2. Do not use the kit after the expiration date.
3. Please check whether all components are complete after opening the package.
All kit components have been formulated and quality control tested to function successfully as a kit. Do not mix or substitute reagents or materials from other kit, performance cannot be guaranteed if utilized separately or substituted.
Materials Required, Not Supplied
1. Microplate reader capable of measuring absorbance at 450 ± 10 nm.
2. High-speed centrifuge.
3. Electro-heating standing-temperature cultivator.
4. Absorbent paper.
5. Distilled or deionized water.
6. Single or multi-channel pipettes with high precision and disposable tips.
7. Precision pipettes to deliver 2 μL to 1 mL volumes.
Safety notes
1. This kit is sold for lab research and development use only and not for use in humans or animals.
2. Reagents should be treated as hazardous substances and should be handled with care and disposed of properly.
3. Gloves, lab coat, and protective eyewear should always be worn, Avoid any skin and eye contact with Stop Solution and TMB. In case of contact, wash thoroughly with water.
$0.00(3) -
mCAP
(Cat No. 2590002)Product Details
Mol wt. 803.4 Appearance Solution (100 mM) Mol Formula C21H30N10O18P3 Purity ≥90% (AX-HPLC) Applications mCAP can be incorporated in transcription by adding a mixture of cap analog and GTP, typically in a 4:1 ratio. As a result, about 80% of the synthesized mRNA will have a 5′ cap, while the remaining 20% will retain a 5′ triphosphate. $0.00(No) -
Mouse T3 (Triiodothyronine) ELISA Kit
(Cat No. 2530001)Product Details
Reagents Quantity Storage Condition 48T 96T Pre-Coated Microplate 6 strips x 8 wells 12 strips x 8 wells -20°C (6 months) Standard (Lyophilized) 1 vial 2 vials -20°C (6 months) Biotinylated-Conjugate (100×) 30 μL 60 μL -20°C (6 months) Streptavidin-HRP (100×) 60 μL 120 μL -20°C (6 months) Standard/Sample Diluent Buffer 10 mL 20 mL 4°C Biotinylated-Conjugate Diluent 5 mL 10 mL 4°C HRP Diluent 6 mL 12 mL 4°C Wash Buffer (25×) 10 mL 20 mL 4°C $0.00(No) -
Mouse T4 (Thyroxine) ELISA Kit
(Cat No. 2530002)Product Details
Reagents Quantity Storage Condition 48T 96T Pre-Coated Microplate 6 strips x 8 wells 12 strips x 8 wells -20°C (6 months) Standard (Lyophilized) 1 vial 2 vials -20°C (6 months) Biotinylated-Conjugate (100×) 30 μL 60 μL -20°C (6 months) Streptavidin-HRP (100×) 60 μL 120 μL -20°C (6 months) Standard/Sample Diluent Buffer 10 mL 20 mL 4°C Biotinylated-Conjugate Diluent 5 mL 10 mL 4°C HRP Diluent 6 mL 12 mL 4°C Wash Buffer (25×) 10 mL 20 mL 4°C TMB Substrate Solution 6 mL 10 mL 4°C (store in dark) Stop Reagent 3 mL 6 mL 4°C Plate Covers 1 piece 2 piece 4°C $0.00(No) -
Mouse TSH (Thyroid Stimulating Hormone) ELISA Kit
(Cat No. 2530003)Product Details
Reagents Quantity Storage Condition 48T 96T Pre-Coated Microplate 6 strips x 8 wells 12 strips x 8 wells -20°C (6 months) Standard (Lyophilized) 1 vial 2 vials -20°C (6 months) Biotinylated-Conjugate (100×) 60 μL 120 μL -20°C (6 months) Streptavidin-HRP (100×) 60 μL 120 μL -20°C (6 months) Standard/Sample Diluent Buffer 10 mL 20 mL 4°C Biotinylated-Conjugate Diluent 6 mL 12 mL 4°C HRP Diluent 6 mL 12 mL 4°C Wash Buffer (25×) 10 mL 20 mL 4°C TMB Substrate Solution 6 mL 9 mL 4°C (store in dark) Stop Reagent 3 mL 6 mL 4°C Plate Covers 1 piece 2 piece 4°C $0.00(No) -
Pname
Product Details
Gene ID gene_id Accession accession Alternative Name alternative_name Species species Source source Description description Accession accession Functions functions Formulation formulation Solubility solubility Appearance appearance Molecular Weight molecular_weight Purity purity Concentration concentration Shipping Condition shipping_condition Storage Condition storage_condition $0.00(No) -
Polymerase Poly (A) Tailing kit (Cat. No. 2510021)
Product Details
E. coli Poly(A) Polymerase (5U/μl) 100 μl 10X PAP Reaction Buffer 600 μl 10mM ATP 600 μl Nuclease-free water 1 ml $0.00(No) -
Protein Quantification Kit (BCA) (Cat. No. 2520007)
Product Details
Reagent A 500 ml room temperature Reagent B 25 ml room temperature BSA (5 mg/ml) 10 ml –20℃ $0.00(No)