3. Please check whether all components are complete after opening the package.
All kit components have been formulated and quality control tested to function successfully as a kit. Do not mix or substitute reagents or materials from other kit, performance cannot be guaranteed if utilized separately or substituted.
Materials Required, Not Supplied
1. Microplate reader capable of measuring absorbance at 450 ± 10 nm.
6. Single or multi-channel pipettes with high precision and disposable tips.
7. Precision pipettes to deliver 2 μL to 1 mL volumes.
Safety notes
1. This kit is sold for lab research and development use only and not for use in humans or animals.
2. Reagents should be treated as hazardous substances and should be handled with care and disposed of properly.
3. Gloves, lab coat, and protective eyewear should always be worn, Avoid any skin and eye contact with Stop Solution and TMB. In case of contact, wash thoroughly with water.
mCAP can be incorporated in transcription by adding a mixture of cap analog and GTP, typically in a 4:1 ratio. As a result, about 80% of the synthesized mRNA will have a 5′ cap, while the remaining 20% will retain a 5′ triphosphate.
